Appeal No. 1999-1393
Application No. 08/242,344
THE AMPA CLASS OF GLUTAMATE RECEPTORS
I. The GLUR1 Subclass:
Appeal No. 1997-3377
Application No. 08/216,326
Claims 31, 34 and 39 are illustrative of the subject matter on appeal and are
reproduced below:
31. A method of assaying a test ligand for the ability to bind to a human CNS
receptor, said method comprising the steps of:
(a) incubating said test ligand with:
(i) a genetically engineered cell that produces an AMPA-
binding human receptor GluR1B having the amino acid
sequence of residues 1-888 of SEQ ID NO:2, said cell
having incorporated expressibly therein a heterologous
DNA molecule encoding the human GluR1B, said cell
further comprising a membrane, wherein said
membrane comprises said human GluR1B, or
(ii) a membrane preparation comprising human GluR1B
derived from said cell,
wherein said incubation is performed under conditions which permit
binding of said test ligand with said human GluR1B; and
(b) determining the extent of binding between said test ligand and
said human GluR1B.
34. The method of claim 33, wherein said test ligand is incubated with
said membrane preparation derived from said human GluR1B-
producing cell.
39. The method of claim 31, wherein said cell comprises a 3.2 kilobase
EcoR1/EcoR1 fragment of the plasmid pBS/human GluR1B (ATCC
75246).
GROUNDS OF REJECTION
Claims 31, 33, 34 and 37-40 are rejected under 35 U.S.C. § 103 over
Puckett in view of Cutting.
We reverse.
Claim 31:
46
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