Appeal No. 1997-3600
Application No. 08/300,447
kt= -1/((PAI) 0-(tPA) 0) ln{(tPA) 0 [(PAI) 0-(tPA) 0+(tPA)]}/(PAI)0(tPA)
wherein (PAI)0 is the initial activity of plasminogen activator inhibitor-1 in
the sample;
(tPA) 0 is the initial activity of tissue plasminogen activator in the sample;
(tPA) is an activity of tissue plasminogen activator at time t; and
the half-life of tissue plasminogen activator in the sample is the value for
t wherein (tPA) is ½ (t-PA) 0;
k is a second order rate constant for a reaction:
k
t-PA + PAI-1 ! t-PA € PAI-1 complex.
22. The method of [c]laim 41 wherein the sample is a body fluid.
25. The method of [c]laim 22 further comprising acidifying the sample,
thereby obtaining an acidified sample.
The references relied upon by the examiner are:
Zeffren et al. (Zeffren), The Study of Enzyme Mechanisms, 54-61 (John Wiley &
Sons 1973)
Chmielewska et al. (Chmielewska), “Determination of Tissue Plasminogen
Activator and Its ‘Fast’ Inhibitor in Plasma,” Clinical Chemistry, Vol. 32, No. 3, pp.
482-485 (1986)
Wun et al. (Wun), “An Inhibitor of Plasminogen Activation from Human Placenta,” J.
Biol. Chem., Vol. 262, No. 8, pp. 3646-3653 (1987)
GROUNDS OF REJECTION1
Claims 22-24, 29-35, 41 and 422 stand rejected under 35 U.S.C. § 103 as
being unpatentable over Wun in view of Zeffren.
1 We acknowledge the examiner’s withdrawal (Supplemental Answer, page 2)
Paper No. 24, mailed March 20, 1997) of the rejection under 35 U.S.C. § 112,
second paragraph. In addition, we note the examiner’s statement (Supplemental
Answer, page 2) that “[c]laims 22-35, [and] 41-42 are currently pending in this
application.” Claims 37-40 were canceled, accordingly the examiner’s Final
Rejection (Paper No. 10, mailed November 8, 1995) of these claims under 35
U.S.C. § 103 is moot.
2
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