Appeal No. 2001-1148 Page 2
Application No. 09/114,552
Claims 11 and 14 are illustrative of the claims on appeal and read as follows:
11. An isolated genetic knock-in primary mammalian adipocyte isolated from a
transgenic mouse, wherein the adipocyte is a progeny of a genetic knock-in cell
made by homologous recombination of a native ob allele with a transgene
comprising a sequence encoding a reporter flanked by flanking sequences which
effect, in conjunction with the cell, the homologous recombination of the transgene
with the native ob allele, whereby the transgene resides on a chromosome in the
transgenic mouse, wherein the expression of the reporter is under the control of
native gene expression regulatory sequences of the native ob allele.
14. A cell-based method for screening for modulators of ob gene expression, the
method comprising steps:
(a) determining a first reporter expression level in a first isolated mammalian
adipocyte according to claim 11;
(b) contacting a second isolated mammalian adipocyte according to claim
11 with a candidate agent under conditions whereby but for the presence of the
agent, the reporter is expressed at the first reporter expression level;
(c) determining a second reporter expression level in the second isolated
mammalian adipocyte; and
(d) comparing the first expression level with the second expression level ,
wherein a difference between the first and second expression levels indicates that
the candidate agent modulates ob gene expression.
The references relied upon by the examiner are:
Tartaglia U.S. 5,741,666 April 21, 1998
Kitamoto et al. (Kitamoto), "Humanized Prion Protein Knock-in Cre-Induced Site-
Specific Recombination in the Mouse," Biochemical and Biophysical Research
Communications, 222, 742-747 (1996).
Kress et al. (Kress), "Hox-2.3 upstream sequences mediate lacZ expression in
intermediate mesoderm derivatives of transgenic mice," Development ,109,
775-786 (1990).
Halaas et al. (Halaas), "Weight-Reducing Effects of the Plasma Protein Encoded by
the obese Gene," Science, Vol. 269, pp. 543-546, 28 July 1995.
Cusin et al. (Cusin), "The ob Gene and Insulin A Relationship Leading to Clues to
the Understanding of Obesity," Diabetes, Vol. 44, pp. 1467-1470 (December
1995).
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