Ex Parte ROTH et al - Page 3



            Appeal No. 1999-1206                                                          Page 3              
            Application No. 08/394,608                                                                        

                   33.  A method for testing the presence of and quantitatively identifying and               
            differentiating microorganisms in a biological test sample containing first and second            
            microorganisms having enzyme activity, comprising the steps of                                    
                   inoculating a solid test medium or substrate capable of forming a solid with said          
            test sample, said test medium comprising a first chromogenic substrate capable of                 
            forming a first water insoluble precipitate of a first color upon reacting with said first        
            microorganism, a second chromogenic substrate capable of forming a second water                   
            insoluble precipitate of a second color contrasting with said first color upon reacting with      
            said second microorganism, and a base medium, said first and second colors being                  
            visibly distinguishable in daylight,                                                              
                   incubating said test medium to produce colonies of said first and second                   
            microorganisms and first and second colored precipitates corresponding to said                    
            colonies,                                                                                         
                   examining said test medium for the presence of colonies of said first                      
            microorganisms having said first color, and the presence of colonies of said second               
            microorganisms having said second color, and                                                      
                   enumerating said colonies of said first and second microorganisms.                         
                   36.  A test medium for detecting the presence of biological material containing            
            first and second microorganisms having enzyme specificity, said test medium                       
            comprising:                                                                                       
                   a base medium for maintenance of said biological material;                                 
                   a first chromogenic substrate forming an insoluble compound of a first color upon          
            reacting with said first microorganism; and                                                       
                   a second chromogenic substrate forming an insoluble compound of a second                   
            color upon reaction with said second microorganism.                                               
                                                                                                             
                   The references relied on by the examiner are:                                              
            Edberg                          4,925,789                      May 15, 1990                     
            Watkins et al. (Watkins), ANovel Compound for Identifying Escherichia coli,@ Appl.                
            Environ. Microbiol., Vol. 54, No. 7, pp. 1874-1875 (1988)                                         
            Ley et al. (Ley), AIndoxyl-β-D-glucuronide, a Novel Chromogenic Reagent for the                   
            Specific Detection and Enumeration of Escherichia coli in Environmental Samples,@                 
            Can. J. Microbiol., Vol. 34, pp. 690-693 (1988)                                                   





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