Appeal No. 1999-1206 Page 3 Application No. 08/394,608 33. A method for testing the presence of and quantitatively identifying and differentiating microorganisms in a biological test sample containing first and second microorganisms having enzyme activity, comprising the steps of inoculating a solid test medium or substrate capable of forming a solid with said test sample, said test medium comprising a first chromogenic substrate capable of forming a first water insoluble precipitate of a first color upon reacting with said first microorganism, a second chromogenic substrate capable of forming a second water insoluble precipitate of a second color contrasting with said first color upon reacting with said second microorganism, and a base medium, said first and second colors being visibly distinguishable in daylight, incubating said test medium to produce colonies of said first and second microorganisms and first and second colored precipitates corresponding to said colonies, examining said test medium for the presence of colonies of said first microorganisms having said first color, and the presence of colonies of said second microorganisms having said second color, and enumerating said colonies of said first and second microorganisms. 36. A test medium for detecting the presence of biological material containing first and second microorganisms having enzyme specificity, said test medium comprising: a base medium for maintenance of said biological material; a first chromogenic substrate forming an insoluble compound of a first color upon reacting with said first microorganism; and a second chromogenic substrate forming an insoluble compound of a second color upon reaction with said second microorganism. The references relied on by the examiner are: Edberg 4,925,789 May 15, 1990 Watkins et al. (Watkins), ANovel Compound for Identifying Escherichia coli,@ Appl. Environ. Microbiol., Vol. 54, No. 7, pp. 1874-1875 (1988) Ley et al. (Ley), AIndoxyl-β-D-glucuronide, a Novel Chromogenic Reagent for the Specific Detection and Enumeration of Escherichia coli in Environmental Samples,@ Can. J. Microbiol., Vol. 34, pp. 690-693 (1988)Page: Previous 1 2 3 4 5 6 7 8 9 10 11 12 13 NextLast modified: November 3, 2007