Appeal No. 1999-1206 Page 3
Application No. 08/394,608
33. A method for testing the presence of and quantitatively identifying and
differentiating microorganisms in a biological test sample containing first and second
microorganisms having enzyme activity, comprising the steps of
inoculating a solid test medium or substrate capable of forming a solid with said
test sample, said test medium comprising a first chromogenic substrate capable of
forming a first water insoluble precipitate of a first color upon reacting with said first
microorganism, a second chromogenic substrate capable of forming a second water
insoluble precipitate of a second color contrasting with said first color upon reacting with
said second microorganism, and a base medium, said first and second colors being
visibly distinguishable in daylight,
incubating said test medium to produce colonies of said first and second
microorganisms and first and second colored precipitates corresponding to said
colonies,
examining said test medium for the presence of colonies of said first
microorganisms having said first color, and the presence of colonies of said second
microorganisms having said second color, and
enumerating said colonies of said first and second microorganisms.
36. A test medium for detecting the presence of biological material containing
first and second microorganisms having enzyme specificity, said test medium
comprising:
a base medium for maintenance of said biological material;
a first chromogenic substrate forming an insoluble compound of a first color upon
reacting with said first microorganism; and
a second chromogenic substrate forming an insoluble compound of a second
color upon reaction with said second microorganism.
The references relied on by the examiner are:
Edberg 4,925,789 May 15, 1990
Watkins et al. (Watkins), ANovel Compound for Identifying Escherichia coli,@ Appl.
Environ. Microbiol., Vol. 54, No. 7, pp. 1874-1875 (1988)
Ley et al. (Ley), AIndoxyl-β-D-glucuronide, a Novel Chromogenic Reagent for the
Specific Detection and Enumeration of Escherichia coli in Environmental Samples,@
Can. J. Microbiol., Vol. 34, pp. 690-693 (1988)
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