Appeal No. 2002-1629 Page 2
Application No. 08/863,121
(b) at least one hydroxylamine salt selected from the group consisting of:
hydrochloride, sulfate, phosphate, and other acid salts; wherein said
multipurpose lyse reagent produces a chromogen having maximum absorbance
between 530 and 550 nm.
The examiner relies on the following references:
Ledis et al. (Ledis) 4,485,175 Nov. 27, 1984
Toda et al. (Toda) 5,250,437 Oct. 05, 1993
Narayanan et al. (Narayanan) 5,336,518 Aug. 09, 1994
Bunn et al. (Bunn), “Hemoglobin: Molecular, Genetic and Clinical Aspects,” W.B.
Saunders Company, pp. 638-644 (1986)
Claims 25, 26, and 28 stand rejected under 35 U.S.C. § 103 as obvious in
view of Toda, Ledis, and Bunn.
Claims 27 and 29 stand rejected under 35 U.S.C. § 103 as obvious in view
of Toda, Ledis, Bunn, and Narayanan.
We reverse.
Background
The “standard methods for determining white blood cell (WBC) counts and
hemoglobin (Hb) determinations traditionally utilize reagents containing potassium
cyanide (KCN) or other cyanide containing compounds. These cyanide
compounds can be hazardous to use because they can produce toxic hydrogen
cyanide (HCN).” Specification, page 1. The standard reagent used in automated
hematology instruments “typically contains ingredients to properly lyse the
erythrocytes to permit accurate leukocyte counting, and a cyanide containing
compound for the formation of a stable chromogen (cyanmethemoglobin) to
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