Appeal No. 2003-0903 Page 2
Application No. 09/534,366
adding said first primer mixture to a second nucleic acid
sample including a first mixture of mRNA to form a first
primer/second nucleic acid sample mixture;
incubating said first primer/first nucleic acid sample mixture
to produce a first population of cDNA;
incubating said first primer/second nucleic acid sample
mixture to produce a second population of cDNA;
adding a PCR amplification reaction second primer mixture
comprising 20 random 10 and I liner primers in equal molar
amounts selected from the group consisting of RT1, RT2, RT3,
RT4, RT5, RT6, RT7, RT8, RT9, RT10, PCR1, PCR2, PCR3,
PCR4, PCR5, PCR6, PCR7, PCR8, PCR9, and PCR10 to said first
population of cDNA to form a second primer/first population of
cDNA mixture;
adding said second primer mixture to said second population
of cDNA to form a second primer/second population of cDNA
mixture;
amplifying said second primer/first population of cDNA
mixture to produce a third population of cDNA;
amplifying said second primer/second population of cDNA
mixture to produce a fourth population of cDNA;
identifying the presence or level of mRNA in said third
population of cDNA, wherein the first mixture of mRNA was
amplified in a single amplification; and
identifying the presence or level of mRNA in said fourth
population of cDNA, wherein the second mixture of mRNA was
amplified in a single amplification.
The examiner relies on the following references:
Liang et al. (Liang ‘672) 5,599,672 Feb. 4, 1997
Heyneker 6,057,100 May 2, 2000
Stratagene Catalog (Stratagene), “Gene Characterization Kits,” p. 39 (1988)
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