Appeal No. 2002-2279 Page 2
Application No. 08/556,667
accomplished by gravity separation alone;
removing at least a portion of the resultant supernatant of said sample portion
including said selected population or subpopulation of said cells of interest from said
particles and said bound population or subpopulation of cells or biological particles;
providing a second plurality of particles having a density of at least two times the
density of the cells of interest and sufficient to provide differential gravity settling, said
particles having bound thereto a reactant which specifically binds to the cells or
biological particles of at least one preselected population or subpopulation not of
interest;
mixing at least a portion of said removed supernatant of said sample portion
including said selected population or subpopulation of cells of interest with said second
plurality of particles to bind said second plurality of particles to said second preselected
population or subpopulation of cells or biological particles without substantially
physically damaging said selected population or subpopulation of cells of interest;
differential gravity settling said second set of particles with said bound population
or subpopulation of cells or biological particles in said supernatant sample portion,
wherein settling is accomplished by gravity separation alone; and
removing at least a portion of the resultant second supernatant including said
selected population or subpopulation of cells of interest from said second set of particles
and said bound population or subpopulation of cells or biological particles and
recovering at least fifty percent of said selected population or subpopulation of cells
originally in said supernatant sample portion.
5. A method of enhancing a selected population or subpopulation of cells of
interest in a fluid sample, comprising:
(a) providing a plurality of particles having a density at least two times the density
of the cells of interest and sufficient to provide differential gravity settling, said particles
having bound thereto at least one reactant which specifically binds to at least one
preselected population or subpopulation of cells or biological particles not of interest;
(b) mixing said sample with said particles to bind said particles to said
preselected population or subpopulation without substantially physically damaging said
population or subpopulation to be enhanced;
(c) permitting said particles and said particles bound to said preselected
population or subpopulation of cells to settle via differential gravity settling, leaving a
supernatant, wherein settling is accomplished by gravity separation alone; and
(d) separating at least a portion of the supernatant comprising said population or
subpopulation to be enhanced from said settled particles and said settled particles
bound to said preselected population or subpopulation not of interest.
The references relied on by the examiner are:
Giaever 4,115,535 Sep. 19, 1978
Grenier et al. (Grenier) 4,788,136 Nov. 29, 1988
Pry et al. (Pry) 5,229,268 Jul. 20, 1993
Falkenburg et al. (Falkenburg), “Selective Removal of Clonogenic Neoplastic B Cells
from Human Bone Marrow Using Anti-HLA-DQ Antibodies and Complement,” Exp.
Hematol., Vol. 14, pp. 101-107 (1986)
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