Appeal No. 2004-2138 Page 3
Application No. 08/765,324
Each of the classes of lipoproteins (LDL, HDL, etc.) in turn includes different
apolipoproteins in different proportions. LDL includes only Apo B-100 as its protein
component, although Apo B-100 is also present in VLDL and intermediate density
lipoproteins (IDL). By contrast, approximately 90% of the apolipoprotein in HDL is Apo
A-I or Apo A-II, while apolipoproteins in the Apo C and Apo E groups are present in all
types of lipoprotein except LDL. Specification, page 2.
“Many epidemiological and clinical studies have shown that increased LDL levels
in the blood are associated with increased risk of CHD [coronary heart disease].” Page
4. “In contrast . . ., individuals with high concentrations of HDL . . . seldom express
symptoms of CHD.” Page 5. The specification discloses “antibodies immunoreactive
with specific epitopes on lipoproteins, such as those on LDL, VLDL and HDL, that
enable rapid and reliable determinations of levels of lipoproteins and/or apolipoproteins
in whole blood, serum or plasma.” Page 14.
In particular, the specification discloses that “[c]onventional ways of producing
MAbs [monoclonal antibodies] to Apo B-100 include immunization of mice with LDL. . . .
However, MAbs produced using LDL as an immunogen tend to be sensitive to
conformational changes of Apo B-100 caused by variations in the lipid composition of
LDL particles.” Page 26. “To obtain an anti-LDL MAb whose binding to LDL particles is
not dependent on variations in LDL composition and/or conformation, mice were
immunized with soluble Apo B-100 which had been delipidized, reduced,
carboxymethylated and, purified by electrophoration in polyacrylamide gels containing 8
M urea (Lee, D.M. et al., Biochim. Biophys. Acta, 666:133-146 (1981)).” Id., page 27.
Page: Previous 1 2 3 4 5 6 7 8 9 10 11 12 13 Next
Last modified: November 3, 2007