Appeal No. 2006-0703 Page 4
Application No. 09/268,437
quiescent solution containing substrate reactive with enzymes bonded to the
analyte binding area.” Reply Brief, pages 4-5.
Claim 11 requires a surface having a plurality of analyte binding areas, a
plurality of working electrodes, each working electrode adjacent to an analyte
binding area and separated from the nearest adjacent analyte binding area by a
distance, all of said binding areas coated with a single quiescent solution
containing substrate reactive with enzymes bonded to analyte binding areas
wherein said device does not have means to mix a sample in said cell.
Cozzette at column 74 teaches a dual analyte combined sensor for
glucose and cholesterol. One electrode is coated with a solution containing
glucose oxidase, while a second, adjacent, electrode is coated with a solution
containing cholesterol oxidase. See id. at Col. 75, lines 1-19. The sensor thus
has a plurality (two) of working electrodes, each working electrode adjacent to an
analyte binding area (the glucose oxidase and the cholesterol oxidase) and
separated from the adjacent binding area by a distance. Once the sensor is
exposed to a test sample containing glucose and cholesterol, it reads on all of
said binding areas coated with a single quiescent solution containing substrate
reactive with enzymes bonded to analyte binding areas wherein said device does
not have means to mix a sample in said cell. Although Cozzette does not
explicitly teach the step of covering the sensor with a test sample, the reference
does teach the use of the biosensors of the invention, see columns 47-52, and
column 73 (assay procedure for analysis of theophylline), and also teaches that
glucose oxidase and cholesterol oxidase produce hydrogen peroxide when
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