Appeal No. 2006-2213 Page 4
Application No. 09/771,151
compound; and
based on said analyzing, selecting Iiposomes of a size that corresponds to
liposomes having no entrapped precipitated compound.
16. A method for preparing Iiposomes which contain a supersaturated
solution of a compound, comprising:
preparing an aqueous supersaturated solution of a compound;
hydrating a Iipid film or a Iipid solution with said supersaturated solution of
the compound to form Iiposomes;
sizing the Iiposomes to selected sizes;
analyzing the Iiposomes at each size for the presence or absence of
precipitated compound; and
based on said analyzing, selecting Iiposomes having a size that
corresponds to liposomes having no precipitated compound.
Thus, claims 1 and 16 are directed to methods of preparing liposomes having
supersaturated solutions of compounds entrapped within them. Claim 1 requires: (a)
selecting a compound showing at least a two-fold solubility increase under specified
conditions; (b) preparing liposomes of selected sizes that contain a supersaturated
solution of the compound; (c) determining whether the liposomes contain precipitated
compound; and (d) “based on the analyzing step,” selecting liposomes of a size having
no precipitated compound in them.
Claim 16 is similar to claim 1 but differs by (a) not requiring the solubility of the
compound to be at least two-fold higher under specified conditions, and (b) requiring
that the liposomes be formed by hydrating a lipid film or solution with a supersaturated
solution of the compound.
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