Appeal No. 2006-2493 Page 2
Application No. 10/126,122
moieties has a molecular mass sum that is distinct from the molecular
mass sum of all other combinations of n peripheral moieties, whereby
members of the mass-coded molecular library which are ligands for the
biomolecule bind to the biomolecule to form biomolecule-ligand
complexes and members of the mass-coded library which are not
ligands for the biomolecule remain unbound;
(b) separating the biomolecule-ligand complexes from the unbound
members of the mass-coded molecular library;
(c) contacting the biomolecule-ligand complexes with the second ligand to
dissociate biomolecule-ligand complexes in which the ligand binds to
the biomolecule at the binding site of the second ligand, thereby
forming biomolecule-second ligand complexes and dissociated ligands;
(d) separating the dissociated ligands and biomolecule-second ligand
complexes; and
(e) determining the molecular mass of each dissociated ligand, wherein
the molecular mass of each dissociated ligand corresponds to a set of
peripheral moieties present in that ligand, thereby identifying a
member of the mass-coded molecular library which is a ligand for the
biomolecule and binds to the biomolecule at the binding site of the
known second ligand for the biomolecule.
The references relied upon by the examiner are:
Jindal et al. (Jindal) WO 97/01755 Jan. 16, 1997
Carell et al. (Carell), “New promise in combinatorial chemistry: synthesis,
characterization, and screening of small-molecule libraries in solution,”
Chemistry and Biology, Vol. 2, No. 3, pp. 171-183 (1995)
Hsieh et al. (Hsieh), “Multidimensional chromatography coupled with mass
spectrometry for target-based screening,” Molecular Diversity, Vol. 2, pp.
189-196 (1996)
van Breeman et al. (van Breeman), “Pulsed ultrafiltration mass
spectrometry: a new method for screening combinatorial libraries,”
Analytical Chemistry, Vol. 69, pp. 2159-2165 (1997)
GROUNDS OF REJECTION
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