Appeal No. 93-3114
Application 07/825,465
linked immunosorbent assay. The response observed
with the lymphocyte transformation is not at clinical
variance with the pathological events noted in
necrobacillosis infections of the bovine.
B. Applicant’s disclosure
Applicant’s specification teaches (Spec., p. 3, lines 5-
32):
The present invention relates to a process for
bacterin preparation in which B-propiolactone (BPL)
is used to inactivate virulent F. necrophorum isolates.
Residual BPL may be hydrolyzed and an adjuvant may then
be added. The bacterin prepared by this process is
useful in the prevention and control of F. necrophorum
in ruminant animals such as sheep, goats and cattle
under normal field conditions.
The present invention also relates to a
B-propiolactone (BPL) killed bacterin prepared from
virulent isolates of Fusobacterium necrophorum which
is useful as an aid in protecting against diseases
caused by F. necrophorum in cloven hoofed animals (i.e.
cattle, sheep, goats, etc.). Examples of such diseases
include Footrot, Liver Abscess, Calf Diphtheria,
Interdigital Dermatitis, etc. Previous attempts at
preparing efficacious bacterins using Fusobacterium
necrophorum have been unsuccessful, probably due to
the fact that critical antigens necessary for induction
of immunity were not preserved by use of inactivation
processes which involved heat or formaldehyde. Applicant
has found, however, through challenge studies conducted
in mice that BPL inactivated, adjuvanted cultures can
protect mice against experimental challenge with
heterologous isolates of F. necrophorum. Field
challenge trials conducted in pregnant sheep and
feedlot cattle have also shown that the BPL inactivated
bacterin of the present invention is effective in
reducing both incidence and severity of ovine and bovine
Footrot.
The present invention can be practiced with any
virulent isolate of F. necrophorum. . . .
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