Interference No. 102,572
(16) Cabilly testified that he conducted a refolding experiment with the material
from the cotransformed heavy and light chain E. coli cells. He grew the cotransformants,
lysed them by sonication, solubilized the pellet with guanidine hydrochloride and incubated
this material overnight at room temperature. He then, dialyzed the reaction mixture against
urea buffer at room temperature followed by dialysis into phosphate buffered saline (PBS).
Cabilly testified that he performed an assay to detect active anti-CEA antibody. He
indicated that he found the heavy chain and light chain protein had recombined to yield
antigen binding activity significantly higher than background. Cabilly, ¶ 8 (CR-40).
(17) Mumford, an employee of Genentech, was responsible for microbial
fermentation optimization of gene products. Mumford, ¶ 2 (CR-35) . The record shows that
on three occasions between December 13, 1982 to March 25, 1983, he or someone in his
lab received and recorded receipt of labeled microbial samples. Mumford, ¶¶ 5-7 (CR-
36). Of specific interest is the receipt on February 2, 1983 of W3110/p10 and W3110/p6 2 2
from Heyneker's laboratory. Mumford, ¶ 7. Mumford recorded:
[T]hese two organisms are E. coli strains, which had been co-transformed
with two plasmids for the co-expression of heavy and light chain of an anti-
CEA antibody. These samples were used to prepare the DMSO stocks
1246-31 and 1246-32, respectively. (¶ 7) (CR-36)
16
(18) Fermentations were run on these two stock solutions. Thereafter, on
February 14, 1983, Mumford recorded the fermentations in CX-18. Mumford, ¶ 13
16The Cabilly et al. brief (page 16) alleges that the fermentations occurred on Feb.
8, 1983. Rey did not give any specific run dates for these fermentations. id.
18
Page: Previous 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 Next
Last modified: November 3, 2007