Appeal No. 95-2215
Application 07/920,013
Independent claim 1 is illustrative of the subject matter on appeal and is reproduced
below:
1. A method for detecting a DNA sequence comprising the steps of:
i) asymmetrically amplifying the DNA sequence so that one strand of the DNA
sequence is amplified to a greater extent than the strands' (sic, strand's) complement
forming an amplified DNA strand;
ii) hybridizing a fluorescently labeled probe to the amplified DNA strand
wherein said fluorescently labeled probe is complementary to a segment of the amplified
DNA strand, the hybridization being effected in solution and forming a fluorescently labeled
hybridized probe;
iii) separating the fluorescently labeled hybridized probe from unhybridized
labeled probe by electrophoresis; and
iv) detecting during electrophoresis the presence or absence of the
fluorescently labeled hybridized probe by fluorescence detection, wherein the presence of
the fluorescently labeled hybridized probe indicates the presence of said DNA sequence.
The references relied upon by the examiner are:
Mullis 4,683,202 Jul. 28, 1987
Gyllensten et al. (Gyllensten), " Generation of single-stranded DNA by the polymerase
chain reaction and its application to direct sequencing of the HLA-DQA locus", Proc. Natl.
Acad. Sci., vol. 85, pages 7652-7656, (October, 1988).
Brumbaugh et al. (Brumbaugh), " Continuous, on-line DNA sequencing using
oligodeoxynucleotide primers with multiple fluorophors", Proc. Natl. Acad. Sci., vol. 85,
pages 5610-5614, (August, 1988).
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