Appeal No. 95-2215
Application 07/920,013
Grounds of Rejection
Claims 1-10 stand rejected under 35 U.S.C. § 103. As evidence of
obviousness, the examiner relies on Gyllensten, Mullis, and Brumbaugh.
We reverse.
Background
At pages 2-3 of the specification, the applicants describe the invention as relating
to a method of detecting a DNA sequence wherein the DNA sequence is asymmetrically
amplified, hybridized, in solution, with a fluorescent labeled probe complementary to a
segment of the amplified DNA sequence, followed by separation of the hybridized and
non-hybridized product using electrophoresis. Applicants state that the labeled DNA is
2
detected during the electrophoresis process in "real time." Applicants also describe the
use of the detection method for detecting the presence or absence of one or more
pathogens in a test sample.
Discussion:
Claims:
Claim 1 is directed to a method of detecting a DNA sequence by asymmetrically
amplifying the DNA sequence, hybridizing, in solution, a fluorescently labeled probe to the
2Applicants define "real time" as "no delay between the time of the electrophoretic
run and the time the results are available." Specification, page 6,
lines 32-35.
3
Page: Previous 1 2 3 4 5 6 7 Next
Last modified: November 3, 2007