Appeal No. 95-2319
Application 07/812,880
Claims 1 and 2 are illustrative of the claims on appeal and are reproduced below:
1. A bioassay for identifying compounds which function as ligands for functional
receptor proteins, said bioassay comprising:
(1) culturing mammalian cells in a medium containing test compound(s);
wherein said cells produce functional receptor protein(s); and wherein said cells have been
infected with recombinant virus comprising DNA encoding an operative hormone
response element operatively linked to DNA encoding reporter, and
(2) monitoring said cells for expression of reporter as an indication of the ability
of test compound to act as a ligand for said receptor protein.
2. A bioassay according to Claim 1 wherein said mammalian cells are
coinfected with recombinant virus comprising:
(a) DNA encoding said functional receptor protein, and
(b) DNA encoding a hormone response element operatively linked to DNA
encoding reporter; wherein said hormone response element is responsive to the functional
receptor protein.
The references relied upon by the examiner are:
Evans et al. (Evans)4,981,784 Jan. 1, 1991
(Filed Nov. 30, 1988)
Stryer, Biochemistry,, W.H. Freeman and Company, San Francisco, page 761, (1975).
Shackleford et al. (Shackleford), "Construction of a clonable infectious, and tumorigenic
mouse mammary tumor virus provirus and a derivative genetic vector" Proc. Natl. Acad.
Sci. USA, vol. 85, pages 9655-9659 (December 1988).
Piccini et al. (Piccini), "Vaccinia Virus as an Expression Vector", Methods in Enzymology,
vol. 153, pages 545-563 (1987).
Jones et al. (Jones), "Isolation of Adenovirus Type 5 Host Range Deletion Mutants
Defective for Transformation of Rat Embryo Cells", Cell, vol. 17, pages 545-563 (1987).
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