Appeal No. 95-2319
Application 07/812,880
quantities of the receptor, the virus further comprise DNA which encode the functional
hormone or hormone-like receptor. Applicant explains that the host cells remain viable and
produce measurable amounts of reporter molecule, upon induction of the hormone
response element by successful ligand complex formation, when a successful ligand
material is tested.
Discussion:
Claims:
Claim 1 is directed to a bioassay for identifying compounds which function as
ligands for functional receptor proteins comprising culturing mammalian cells, which have
been infected with recombinant virus, in a medium containing the test compound or
compounds. The recombinant virus comprise DNA which encode an operative hormone
response element operatively linked to DNA encoding reporter. The cultured cells are
monitored for expression of the reporter, which is indicative of the ability of the compound
being tested to act as a ligand for the receptor peptide. Claim 2 is directed to a bioassay
wherein the mammalian host cells are co-infected with recombinant virus comprising DNA
encoding the functional receptor and DNA encoding a hormone response element
operatively linked to a DNA encoding reporter.
The rejections under 35 U.S.C. § 103
Claims 1-3, 9, 15, 16, and 21:
Claims 1-3, 9, 15, 16, and 21 stand rejected under 35 U.S.C. § 103 as obvious
over Evans in view of Stryer.
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