Appeal No. 95-4342
Application 07/938,832
Claims 23 and 15 are illustrative of the subject matter on appeal and read as
follows:
15. A kit for performing monosaccharide linkage structural analysis of
carbohydrates, said kit comprising, a carbohydrate derivatizing agent, a glycosidic bond
hydrolyzing agent and a fluorophore label.
23. A method of analyzing structural relationships between monosaccharides
of a carbohydrate comprising:
a) derivatizing a carbohydrate with a carbohydrate derivatizing agent which
covalently binds to the free functional groups of the carbohydrates to form a derivatized
carbohydrate;
b) converting said derivatized carbohydrate into derivatized monosaccharides by
breaking the glycosidic bonds of said derivatized carbohydrate;
c) forming labeled derivatized monosaccharides by reacting said derivatized
monosaccharides with a fluorescence labelling compound;
d) separating said labeled derivatized monosaccharides by electrophoresis; and
e) identifying said labeled derivatized monosaccharides by comparing the
electrophoresis mobility of said labeled derivatized monosaccharides to a
monosaccharide standard, said monosaccharide standard comprising mono-saccharides
derivatized with said carbohydrate derivatizing agent and labeled
with said fluorescence labelling compound.
The references relied upon by the examiner are:
Lindberg, “Methylation Analysis of “Complex Carbohydrates: General Procedure and
Application for Sequence Analysis”, Methods in Enzymology, Vol. 50, pp. 3-33 (1978).
Levery, “Microscale Methylation Anaysis of Glycolipids Using Capillary Gas
Chromatography -- Chemical Ionization Mass Fragmentography with Selected Ion
Monitoring,” Methods in Enzymology, Vol. 138, Part E. pp. 13-25 (1987).
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