Appeal No. 95-4481
Application No. 07/911,667
Claims 1 through 3 are illustrative of the subject matter on appeal and read as
follows:
1. A method of assaying for antibodies to human double-stranded DNA in a liquid,
human-serum specimen, said method comprising the steps of:
forming a first incubation mixture of said specimen and a solid phase
immunoadsorbent having immobilized thereon animal anti-human IgG antibody including
F(ab')2 fragment specific for IgG Fc;
incubating said first mixture under conditions and for a period of time sufficient for
Fc in human IgG in said specimen to become bound by said F(ab')2 fragment in said
animal anti-IgG antibody;
forming a second incubation mixture of said first mixture and digoximenin-labelled
synthetic double-stranded DNA under such conditions and for a period of time sufficient to
bind to said immunoadsorbent any anti-DNA antibodies which may be present in the
serum so as to form a double-stranded DNA antibody that has substantially no single
stranded or denatured components;
detecting the amount of digoxigenin labelled synthetic double-stranded DNA bound
to said immunoadsorbent; and
relating the amount of bound labelled synthetic double-stranded DNA detected to a
predetermined quantitative relationship between the amount of labelled double-stranded
DNA and the amount of animal anti-human IgG antibody to determine the amount of human
IgG in said specimen.
2. A method of assaying for antibodies to human double-stranded DNA in a human
serum specimen, said method comprising the steps of:
capturing the anti-double-stranded DNA portion of IgG in said human serum
specimen by the Fc part of a molecule using solid phase immobilized F(ab')2 fragment of
anti-human IgG specific for Fc;
2
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Last modified: November 3, 2007