Appeal No. 95-4481
Application No. 07/911,667
incubating the captured IgG with synthetic double-stranded DNA tagged with a
moiety from which a signal proportional to the quantity of said synthetic double-stranded
DNA can be elicited;
eliciting said signal; and
quantifying the amount of antibody to double-stranded DNA in accordance with the
elicited signal.
3. A method as defined in claim 2 wherein said
captured IgG is incubated with synthetic double-stranded DNA tagged with alkaline
phosphatase, and said elicited signal is proportional to the quantity of alkaline
phosphatase tagging said synthetic double-stranded DNA.
The references relied on by the examiner are:
Litman et al. (Litman) 4,391,904 July 5, 1983
Denise Pollard-Knight (Pollard-Knight), "Current Methods In Nonradioactive Nucleic Acid
Labeling and Detection," 2 Technique-A Journal of Methods in Cell and Molecular Biology,
no. 3, 112-132 (June 1990).
Richard M. Pope, et al. (Pope), "IgG Rheumatoid Factor: Analysis of Various Species of
IgG for Detection by Radioimmunoassay," 97 Journal of Methods. no.6, 842-853 (1981).
Woodruff Emlen, et al. (Emlen), "A New ELISA for the Detection of Double-Stranded DNA
Antibodies," 132 Journal of Immunological Methods, 91-101 (1990).
Claims 1 and 2 stand rejected under 35 U.S.C. § 103 as unpatentable over Litman
in view of Pope and Emlen and claims 3 through 7 stand rejected under 35 U.S.C. § 103
as unpatentable over Litman in view of Pope and Emlen and further in view of Pollard-
3
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