Ex parte LIGLER et al. - Page 2




          Appeal No. 1995-4188                                                        
          Application 07/860,965                                                      


          claims remaining in the application.                                        




                                    THE INVENTION                                     
               Appellants claim a flow immunosensor which contains,                   
          inter alia, a labeled antigen which saturates the antigen-                  
          binding sites of an immobilized antibody and is capable of                  
          being displaced by a target.  Claim 40 is illustrative and                  
          reads as follows:                                                           
               40.  A flow immunosensor for performing a real-time                    
          analysis of a sample which is to be analyzed and which is not               
          yet present, said flow immunosensor consisting essentially of;              
               an exchanger including:                                                
                    a chamber having an inlet port for sample                         
          introduction and an outlet port,                                            
                    a support medium, comprising a bed having a volume                
          of        0.1 to 0.5 ml, in the chamber,                                    
                    an antibody immobilized on the medium wherein the                 
               antibody recognizes with specificity and sensitivity a                 
               target,                                                                
                    a labelled antigen complexed to said immobilized                  
                    antibody and capable of being displaced by the                    
          target, said labelled antigen saturating the antigen-binding                
          sites of       said antibody;                                               
                    a detection apparatus connected to the exchanger via              

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