Appeal No. 1996-1618
Application No. 07/876,804
amended subsequent to the final rejection, which are all of the claims pending in this
2
application. Claims 23, 24 and 29 are illustrative and read as follows.
23. A device for binding a specific chemical in a liquid which device
comprises:
a piezoelectric crystal substrate having a surface layer of silicon
dioxide containing hydroxyl groups;
a layer of coupling agent bound to said substrate, said agent having a
functional group wherein said coupling agent is a silyl compound of the
formula:
R SiX
n (4-n)
wherein R is a nonhydrolyzable organic radical containing a primary alcohol
group, X is a hydrolyzable moiety selected from the group consisting of
chloro, amino, alkoxy, and acyloxy, and n is an integer from 1 to 3;
a single ligand-binding layer attached to said coupling agent by
means of said functional group on said coupling agent, said ligand-binding
layer having binding sites thereon for binding a ligand-bearing substance
thereto;
a single ligand-bearing layer bound to said ligand-binding layer, said
ligand-bearing layer being capable of selectively binding said specific
chemical.
24. A device as in Claim 23 wherein said ligand-binding substance
is selected from the group consisting of avidin, streptavidin, acetylated
avidin, acetylated streptavidin, succinylated avidin, succinylated streptavidin,
genetically engineered avidin with intact biotin binding sites [sic] genetically
engineered streptavidin with intact biotin binding sites, modified avidin with
intact biotin binding sites, and modified streptavidin with intact biotin binding
sites; and said ligand-bearing substance is a biotinylated compound.
29. A device as in Claim 23 wherein said ligand-bearing substance
is a lectin, said lectin having binding affinity for selected sugars.
2The amendment filed March 14, 1994 (Paper No. 16) amending claims 17, 22 and 23 and
cancelling claims 19-21 was entered by the examiner in the advisory action mailed April 7, 1994 (Paper No.
18). The amendment filed April 17, 1995 (Paper No. 26) amending claim 29 was entered by the examiner in
the supplemental examiner’s answer mailed July 10, 1995 (Paper No. 28, page 2).
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