Appeal No. 1996-1618
Application No. 07/876,804
ligand-bearing layer bound to said ligand-binding layer, said ligand-bearing layer being
capable of selectively binding a specific chemical, e.g., a single layer of biotinylated
antibody which antibody selectively binds said specific chemical.
1. Rejection of claims 17, 18, 22-25, 27 and 28 over Bastiaans, Wilchek and Katz
Bastiaans discloses a piezoelectric crystal substrate having a GOPS coupling layer
and antigen or antibody attached directly to the coupling layer (col. 2, lines
45-59). Wilchek is a review article which describes avidin-biotin complex as a general
mediator in a variety of bioanalytical applications,
mainly for isolation (affinity chromatography), localization (affinity
cytochemistry, cell cytometry, and blotting technology), and diagnostics (for
immunoassay and histopathology and as gene probes). More recently,
current usage of the system has been extended to include various other
areas, such as hybridoma technology, bioaffinity sensors, affinity targeting,
and drug delivery, as well as crosslinking, immobilization, and fusogenic
studies. [Page 1, col. 1.]
Katz describes a solid phase support, coated in whole or in part with avidin, and upon
which biotinylated antibody is coated in selected areas (Fig. 1; col. 2, lines 19-24) useful in
an enzyme immunoassay. For example, biotinylated anti-HCG antibody is reacted with an
avidin-coated support, followed by contact with a sample suspected of containing HCG
and then by contact with a labeled anti-HCG antibody to determine HCG based upon the
amount of detectable label bound to the support (col. 1, line
59 - col. 2, line 9).
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