Ex parte SPANA et al. - Page 2




               Appeal No. 1996-2210                                                                                             
               Application 08/129,722                                                                                           



                      (a)  a first coding region, which codes for glutathione-S-transferase, operatively                        
               connected to a baculovirus promoter,                                                                             
                      (b)  a second coding region in-frame with the first coding region, and                                    
                      (c)  a restriction region containing at least one restriction site downstream of the first                
               coding region, into which the second coding region is inserted;                                                  
               wherein a fusion protein encoded by the first and second coding regions would be produced                        
               by expression of the vector.                                                                                     
                      23.  An expression vector, comprising:                                                                    
                      (a)  a first coding region, which codes for glutathione-S-transferase, operatively                        
               connected to a promoter, and                                                                                     
                      (b)  a restriction region downstream of the first coding region having the sequence of                    
               SEQ. ID No.:5, which allows for insertion of a second coding region in-frame with the first                      
               coding region.                                                                                                   

                      The references relied on by the examiner are:                                                             
               Ausubel et al. (Ausubel), Short Protocols In Molecular Biology, Second Edition, AA                               
               Compendium of Methods from Current Protocols in Molecular Biology,@ John Wiley & Sons,                           
               pp. 16-28-16-48 (1992).                                                                                          
               PCT (Laroche)                                WO 91/16353                   Oct. 31, 1991                         
                      The issues presented for review are:                                                                      
                      (1)  whether the examiner erred in rejecting claims 1 through 4, 6 through 17, and 22                     
               under 35 U.S.C. ' 103 as unpatentable over Ausubel; and                                                          


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