Appeal No. 1996-3973
Application No. 08/048,657
APPENDIX
1. A test strip, comprising:
a first layer comprising an indicator reagent impregnated into a porous
substrate;
a second layer, overlying the first layer, comprising a reactive reagent, the
indicator reagent and the reactive reagent being jointly reactive with glucose to
produce a measurable output in the event that glucose is present in the liquid
sample;
a third layer, overlying the second layer on a side opposite the first layer,
comprising a spreading agent that promotes the spreading of a liquid upon the
third layer; and
an inert backing overlying the third layer, the backing having an aperture
therethrough to the third layer, the aperture being in registry with the first layer,
the second layer, and the third layer.
2. The test strip of claim 1, wherein the porous substrate is a nylon
membrane.
3. The test strip of claim 1, wherein the indicator is DMAB (3-
dimethylaminobenzoic acid).
4. The test strip of claim 1, wherein the reactive reagent is selected from
the group consisting of glucose oxidase and peroxidase.
5. The test strip of claim 1, wherein the spreading agent is selected from
the group consisting of cellulose acetate and diatomaceous earth.
6. A test strip, comprising:
a first layer comprising an indicator reagent impregnated into a porous
substrate;
a second layer, overlying the first layer, comprising a reactive reagent and
a substance promoting the spreading of a liquid upon the second layer, the
indicator reagent and the reactive reagent being jointly reactive with glucose to
produce a measurable output in the event that glucose is present in the liquid
sample; and
an inert backing overlying the second layer, the backing having an aperture
therethrough to the second layer, the aperture being in registry with the first layer
and the second layer.
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