Appeal No. 1996-3973
Application No. 08/048,657
means for determining the presence of an active species in a liquid
sample, and
spreadability enhancing material means for causing a droplet of
liquid to spread across a top surface of the reaction unit layer structure; and
a backing having an aperture therein, to which the reaction unit is affixed
with the top surface contacting the backing, the spreadability enhancing material
means serving to cause a droplet of liquid placed into the aperture to spread across
the full extent of the aperture.
14. A method for preparing a test strip, comprising the steps of:
providing a porous substrate;
providing an indicator solution containing the indicator DMAB [3-
dimethylaminobenzoic acid], the indicator solution having a sufficiently low
viscosity that it is penetrable into the porous substrate;
introducing the indicator solution into the porous substrate;
drying the indicator solution within the porous substrate;
providing a reactive reagent solution containing a reagent selected from
the group consisting of glucose oxidase and peroxidase, the solution having a
sufficiently high viscosity that it is not penetrable into the porous substrate;
applying the reactive reagent solution as a reactive reagent layer to a
surface of the porous substrate;
drying the reactive reagent within the reactive reagent layer; and
attaching the porous substrate to a backing having an aperture
therethrough, with the applied layer adjacent to the backing and the porous
substrate remote from the backing.
15. The method of claim 14, wherein the step of providing a reactive
reagent solution includes the step of
mixing a spreading solution into the reagent solution.
16. The method of claim 14, including the additional steps, after the step
of drying the reactive reagent and before the step of attaching the porous substrate,
of
providing a spreading solution containing a spreading agents; and
applying the spreading solution as a spreading layer to the reactive reagent
layer.
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