Appeal No. 1996-4119
Application No. 08/261,406
recovering a fraction comprising [.alpha]1 proteinase inhibitor from
the anion-exchange chromatography medium;
applying I1-proteinase inhibitor recovered from the anion-
exchange chromatography medium to a metal chelate medium; and
recovering a fraction comprising I1-proteinase inhibitor from
the metal chelate medium.
5. A process as recited in claim 1 wherein the ZnCl2 is added to the impure
protein fraction to a concentration of 0.25 to 0.75 mM.
The references relied upon by the examiner are:
Neurath et al. (Neurath) 4,540,573 Sep. 10, 1985
Bollen et al. (Bollen) 4,629,567 Dec. 16, 1986
Protein Purification Methods, a practical approach 154-170 (E. L. V. Harris & S.
Angal eds., IRL Press, Oxford 1989) (Harris)
Bischoff et al., “Purification and Biochemical Characterization of Recombinant I1-
Antitrypsin Variants Expressed in Escherichia coli,” Vol. 30, pp. 3464-3472 (1991)
Ng et al. (Ng), “Plasma Protein Recovery from Spent Tissue Culture Fluid,”
Biotechnology Letters, Vol. 13(4), pp. 261-264 (1991)
Yip et al. (Yip), Immobilized Metal Ion Affinity Chromatography, in Methods in
Molecular Biology 17-31 (A. Kenney & S. Fowell eds, The Humana Press Inc.,
1992)
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