Appeal No. 1997-1668
Application No. 08/361,024
1. A kit comprising reagents for amplification of at least one target
sequence comprising at least one region having a defined nucleic acid
sequence, the kit comprising at least one Blocker moiety, at least one
Primer moiety, and at least one End-Run moiety, where the Blocker moiety
is capable of hybridizing to the nucleic acid sequence, the Primer moiety is
capable of hybridizing to the nucleic acid sequence such that the Primer
moiety abuts the hybridized Blocker moiety or is capable of extending to the
hybridized Blocker moiety, and the End-Run moiety comprises a sequence
which is complementary to at least a portion of the Blocker moiety.
[Emphasis added.]
5. A kit comprising components for conducting a reaction for
amplifying or detecting a target sequence of a polynucleotide, said reaction
comprising the steps:
a) treating said polynucleotide with at least three oligonucleotide
moieties wherein said at least three oligonucleotide moieties include:
i) a first oligonucleotide moiety comprising a
nucleotide sequence complementary to and capable of
hybridizing to the polynucleotide;
ii) a second oligonucleotide moiety complementary to
and capable of hybridizing to the polynucleotide such that the
second oligonucleotide moiety abuts the first oligonucleotide
moiety when the first oligonucleotide moiety is hybridized to
the polynucleotide or such that the second oligonucleotide is
capable of extending to the first oligonucleotide moiety when
the first oligonucleotide moiety is hybridized to the
polynucleotide; and
iii) a third oligonucleotide moiety comprising a
sequence which is complementary to at least a portion of the
first oligonucleotide moiety;
b) providing conditions for hybridizing the first moiety and the second
moiety to the polynucleotide;
c) providing conditions for ligating the hybridized first moiety to the
hybridized second moiety to form a ligation product; and
d) providing conditions for hybridizing and chain extending the third
moiety,
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