Appeal No. 1997-1770
Application No. 08/235,488
17. A sterile medium for use in detecting coliform
bacteria and E. coli, said medium comprising:
(a) a $-D-galactosidase substrate;
(b) a carbohydrate metabolizable by a plurality of
coliform species but not metabolize by E.
coli; and
(c) salts.
The following references of record are relied upon by the
examiner as evidence of obviousness:
Edberg 4,925,789 May 15,
1990 Roth et al. (Roth) 5,210,022 May
11, 1993
"Dehydrated Culture Media and Reagents for Microbiology, in
Difco Manual," 203-4 (10th ed., Detroit MI, DIFCO Labs, 1984).
Chang, G., "Tactics for Combining the Coliform and Indole
Tests: Simple Media for Both Total Coliforms and Escherichia
Coli," 53 Journal of Food Protection 910 (October 1990).
Chang, G., et al. (Chang), "Tryptophan and Galactoside (TAG)
Media: Simple and Specific Ways to Enumerate E. coli and Total
Coliforms in Water and Food," 90 Society of Microbiology, no.
0, 290, abstract Q-12 (1990).
Bainbridge, B. et al. (Bainbridge) "Improved methods for the
detection of $-galactosidase activity in colonies of
Escherichia Coli using a new chromogenic substrate: VBzTM-gal
(2-(2-(4-($-D-galactopyranosyloxy)-3-methoxyphenyl)-vinyl)-3-
methylbenzothiazolium toluene-4-sulphonate 80 FEMS
Microbiology Letters, 319-24 (1991).
Atlas, R. et al. (Atlas), "Handbook of Microbiological Media,"
132, 178 (Boca Raton, FL, CRC Press, no date available).
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