Appeal No. 1997-2389
Application No. 08/987,233
microbe is randomly distributed on the membrane material associated with said
receptacles,
filtering the aliquots to collect on the membrane material microbes contained in the
liquid sample and
sequentially performing in a single assay
1. a first test to determine the presumptive presence of the specific microbe
and to confirm the presence of the microbe, which first test comprises:
(a) contacting the membrane material with a selective [sic] medium permitting
growth of the microbes collected, said medium including metabolizable substrates;
(b) incubating the membrane materials so that the microbes multiply;
(c) analyzing the medium for a metabolic by product which indicates the
presumptive presence of the specific microbe to be determined and for the presence of
another metabolic by-product which confirms the presence of said microbe; and
(d) removing the non-selective medium from the membrane material and
2. a second test to completely determine and quantify the presence of the
microbe, which second test comprises:
(a) contacting the microbe collected on the membrane material with a
predetermined amount of a detectable reagent specific for the microbe to be determined
under conditions permitting formation of complexes between the reagent and the microbe
and
(b) determining the amount of complex formed and thereby the amount of the
specific microbe originally present in the liquid sample.
29. The method of claim 27 wherein the reagent is a polynucleotide
complementary to a gene of the microbe.
43. A method for analyzing a liquid sample to quantitatively determine the
presence of a viable specific microbe which comprises
2
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