1. A method for the multi-parameter analysis of cells in a body fluid wherein said
method comprises the steps of:
1) taking a fluid sample from an individual;
2) adding to said sample a nucleic acid dye;
3) adding to said sample a first fluorescently labelled monoclonal antibody and a
second fluorescently labelled monoclonal antibody, wherein said first antibody recognizes
an antigen that is differentially expressed on said cells and wherein said second antibody
recognizes an antigen that is differentially expressed on erythrocyte precursors and
proliferating cells, and further wherein the fluorescent labels on said antibodies have peak
emission spectra different from each other and from said nucleic acid dye;
4) analyzing said cells in an instrument capable of detecting and recording at least
three channels of fluorescence and at least two channels of light scatter for each of the
cells in said sample, wherein erythrocytes, mature reticulocytes, immature reticulocytes,
mature nucleated erythrocytes, immature nucleated erythrocytes, progenitors,
megakaryocytes, neutrophils, eosinophils, basophils, monocytes, lymphtocytes, stromal
and epithelial cells are identified, characterized, and enumerated.
5. The method of claim 1 wherein the first monoclonal antibody is an anti-CD45
monoclonal antibody.
6. The method of claim 1 wherein the second monoclonal antibody is an anti-CD71
monoclonal antibody.
8. The method of claim 1 wherein a third fluorescently labelled monoclonal antibody
is added wherein said antibody recognizes antigens differentially expressed on platelets.
The references relied on by the examiner are:
Loken et al. (Loken) 5,047,321 Sep. 10, 1991
Basch et al. (Basch), “Expression of CD4 by human megakaryocytes,” Proc. Natl. Acad.
Sci., USA, Vol. 87, pp. 8085-089 (Oct. 1990)
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