Appeal No. 1997-2391
Application 08/239,265
myeloid cells and non-hematopoietic cells (i.e., stromal and epithelial cells).
(Specification, page 2).
Kansas describes the pattern of expression of three classes of leukocyte adhesion
molecules during normal differentiation of monocytes, granulocytes, and erythrocytes in
humans: the CD11/CD18 heterodimers (including CD11a/CD18, the leukocyte function-
associated antigen 1 (LFA-1)), the CD44 family, and the LAM-1 (leukocyte adhesion
molecule 1) molecule(s). Differential expression of transferrin receptor/CD71 and CD45
was noted in the course of investigating the down regulation of CD44 during erythroid
development:
Although the absence of any markers specific for glycoG erythroid cells make
analysis of the phenotype of these cells difficult, the data described below
hi
indirectly suggest that these early committed erythroid cells are CD44 LFA-
1GLAM-1G. No cells coexpressing glyco and either LFA-1 or LAM-1 were
detectable . . . , consistent with the loss of these markers being an early
event in erythropoiesis. In contrast, CD44 was expressed at high levels on a
+ + hi
subset of glyco cells; further analysis showed that these glyco CD44 cells
coexpressed CD45, a marker found on all leukocytes and early erythroid
+
cells. Glyco cells that expressed intermediate levels of CD44 were
+ +
transferrin receptor/CD71 . Glyco cells expressing neither CD45 nor CD71
expressed low levels of CD44, similar to that found on normal, circulating
RBC. Thus, CD44 expression declines gradually in a stepwise fashion
during normal erythropoiesis. (Kansas, page 2486, citations and references
to figures omitted).
+ + int
In addition, Figure 4b of Kansas shows that “most glyco CD71 cells are CD44 , but a
+ + hi
minor subset of the glyco CD71 are CD44 .”
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