Appeal No. 1997-2926
Application No. 08/376,299
APPENDIX
1. A method for treating an organic industrial waste to
degrade the waste, which comprises:
(a) providing dried cells of a microorganism which is
anaerobic and can degrade the waste admixed with a dried
growth medium for the microorganism containing an alkali metal
nitrate in an amount between about 15 and 45 percent by weight
of the growth medium in a sealed container with folds which
collapses the container for shipment of multiple containers in
a single box and with a closeable opening on the container,
wherein the folds are horizontally opposite each other
(b) shipping the collapsed container near a site where
the microorganism is to be introduced into the waste;
(c) providing the collapsed container in a containment
means so that the folds are below the closeable opening and
the folds engage the containment means as the container is
filled;
(d) filling the container through the opening with water
so as to unfold the container and to form a liquid growth
medium from the dried growth medium admixed with the
microorganism;
(e) holding the microorganism in the liquid growth medium
in the container for a period of time of up to about 32 hours
and at a temperature so that the microorganisms grow and
increase in cell number and wherein the alkali metal nitrate
and the microorganism inhibit any contaminant microorganisms
in the liquid growth medium; and
(f) adding the microorganism along with the liquid growth
medium to the industrial waste in a treatment plant, a lagoon,
a tank or a soil, wherein the waste is degraded.
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