Appeal No. 1997-3221
Application No. 08/249,241
Appeal No. 2000-0440
Application No. 08/217,704
Claim 14 is illustrative of the subject matter on appeal and is reproduced
below:
14. A method of assaying a candidate ligand for binding interaction with a
human NR2A protein, which comprises the steps of:
(1) incubating the candidate ligand under appropriate conditions with
a cell that has been mutated to produce a human NR2A protein,
said cell having incorporated expressibly therein a
heterologous polynucleotide that encodes a modulatory protein
selected from the group consisting of NR2A-1 having the amino
acid sequence of SEQ ID NO:2 and NR2A-2 having the amino
acid sequence of SEQ ID NO:2 wherein the lysine at position 270
is replaced by glutamic acid,
or with a membrane preparation containing said NR2A protein,
and then
(2) determining the extent of binding between the human NR2A
protein and the candidate ligand.
GROUNDS OF REJECTION
Claims 14, 21 and 22 are rejected under 35 U.S.C. § 103 as being
unpatentable over Monyer in view of McNamara, Blackstone, Schofield, Grenningloh
and Puckett.
Claims 23 and 34 are rejected under 35 U.S.C. § 103 as being unpatentable
over Monyer in view of McNamara, Blackstone, Schofield, Grenningloh and Puckett
as applied to claims 14, 22, and 23 above and further in view of Durand.
We reverse.
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