Appeal No. 1997-3542
Application No. 08/192,507
discloses the antibodies may be fluorescein-labeled. Kung, abstract and page 874,
column 1. Kung provides rat anti-mouse IgM antibodies bind to an allotypic determinant
of mouse IgM. The examiner acknowledges that neither Julius nor Kung teach the
referenced monoclonal antibodies linked to a cytotoxic moiety. Answer, page 10.
The examiner relies on Lambert for the production of several different
immunotoxins specific for surface markers on B cells and other lymphoid cells. The
immunotoxins are prepared by conjugating monoclonal antibodies to cytotoxic moieties,
including the ribosome inactivating protein, gelonin, and pokeweed anti-viral protein.
Lambert, page 12035. Lambert describes seven different monoclonal antibodies of the
IgG class which bind to four different antigens on human B cells and other lymphoid cells
which may be used to prepare the immunotoxins. Lambert suggests that receptor-
mediated endocytosis of an antigen/immunotoxin complex may be essential for
cytotoxicity and provides evidence that CALLA and Ia antigens are internalized by B cells
and that the B1 antigen shows no tendency of being internalized. Lambert, page 12036.
The lack of cytotoxicity of the anti-B1 immunotoxins is attributed to the fact that anti-B1
immunotoxins are not internalized and are not transported into the cell. Lambert does not
specifically suggest that the antibody linked to the immunotoxin be specific for IgM antigen
on B cells. The disclosure of Taylor is cumulative in some respects to that of Lambert,
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