Appeal No. 1999-1393
Application No. 08/242,344
2555, Materials and Methods) a human placental DNA library was screened for
GluR1-4 by hybridization to radiolabeled probes of rat GluR1-4 cDNA. McNamara
(page 2556, Results) reports the isolation of cosmid clones containing portions of
the putative human GluR genes by hybridization under stringent conditions with the
homologous cDNA obtained from rat, and that “[u]nder these conditions, the cosmid
clones hybridized to radiolabeled cDNA of mainly one GluR cDNA.” McNamara
(page 2556, Results) further reports that “[i]n every instance, the homology of the
human GluR sequence was higher with its respective rat cDNA than with rat cDNAs
encoding other GluRs.” This data is illustrated in Table 1 (page 2557). McNamara
states (page 2557, Discussion) “[t]he results of selective hybridization and partial
sequence analysis support the conclusion that the genomic clones isolated
represent human homologs corresponding to rat GluR1-4.” McNamara does not
teach a nucleotide or amino acid sequence of GluR4. Additionally, McNamara
does not teach a GluR4B receptor.
However, Sommer ’90 is cited by the examiner for teaching flip and flop
forms of GluRA-D. We note that McNamara (page 2555, column 2) recognizes a
correspondence between the nomenclature of human GluR1-4 and rat GluRA-D.
Thus rat GluRD corresponds to human GluR4. We also note that Sommer ’90 teach
(Figure 1, page 1581) the “complete nucleotide sequences encoding the flop-
containing polypeptides are deposited at EMBL/GenBank under accession
numbers … M36421 (GluR-D) and the corresponding flip versions under …
M38063 (GluR-D).”
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