Appeal No. 1995-3965
Application No. 07/719,005
Claims 1 and 9 are illustrative of the subject matter on appeal and are
reproduced below:
1. A process for detecting the presence of different selected standard
microorganisms in a sample by reverse genome probing, wherein said
process comprises the steps of:
(A) preparing a master solid surface having affixed thereto isolated
spots of genomic deoxyribonucleic acid from each of said
different selected standard microorganisms by:
(i) obtaining an individual culture of each of said selected
standard microorganisms from an environment of
interest,
(ii) isolating a genomic deoxyribonucleic acid
preparation from each of said individual cultures, and
(iii) affixing each of said genomic deoxyribonucleic acid
preparation from step (Aii) to said master solid
surface,
(B) preparing a reverse genome probe from said sample by:
(i) isolating a total nucleic acid preparation from said
sample by:
(ii) labeling said total nucleic acid preparation,
(C) contacting said master solid surface from step (A) with said
reverse genome probe from step (B) under conditions permitting
hybridization,
(D) washing said master solid surface resulting from step (C) so as to
remove non-hybridized reverse genome probe, and
(E) detecting hybridized reverse genome probe.
9. A master solid surface for use in detecting the presence of different
selected standard microorganisms by reverse genome probing, said
master solid surface having affixed thereto isolated spots of genomic
deoxyribonucleic acid from each of said selected standard
microorganisms, wherein said different selected standard
microorganisms are isolated from an environment of interest.
The references relied upon by the examiner are:
Hitzman 4,540,052 Sep. 10, 1985
Kimmel, “Selection of Clones from Libraries: Overview,” Methods in Eznymology,
Vol. 152, pp. 393-399 (1987)
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