Appeal No. 1997-2364
Application 08/137,086
1. A method for the preparation of selected phosphopeptides comprising the steps
of:
completely digesting a soluble monovalent cation salt of casein in solution,
introducing a di or trivalent metal ion to cause aggregation of at least the selected
phosphopeptides in said digested solution, and
diafiltering the digested solution containing the aggregating ion through a filter
having a molecular weight exclusion limit selected to retain at least said aggregated
phosphopeptides while passing the bulk of the remaining phosphopeptides in a filtrate,
wherein the metal ion concentration during diafiltration is maintained at a level effective to
maintain the aggregated phosphopeptides in aggregate form.
2. The method of claim 1, wherein the selected phosphopeptides are
anticariogenic phosphopeptides and the molecular weight exclusion limit adopted during
the filtering step substantially falls within the range 10,000 to 20,000.
7. A method for the preparation of selected phosphopeptides having
anticariogenic and other activities, comprising the steps of:
completely digesting a soluble monovalent cation salt of casein in solution with a
proteolytic enzyme,
adding a mineral acid to the solution to adjust the pH to about 4.7,
removing any precipitate produced,
adding CaCl to the digested solution to a level of about 1.0% w/v to cause
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aggregation of at least the selected phosphopeptides in said digested solution, and
separating the aggregated phosphopeptides from the solution by filtration including
diafiltration with the CaCl containing solution through a filter having a molecular weight
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exclusion limit lying substantially within the range 10,000 to 20,000 while passing the bulk
of the remaining phosphopeptides and non-phosphorylated peptides and solution in a
filtrate, wherein the metal ion concentration during diafiltration is maintained at a level
effective to maintain the aggregated phosphopeptides in aggregate form.
10. Phosphopeptides when produced by the method of claim 1.
12. The method of claim 1, wherein the diafiltering step further comprises
diafiltering the digested solution with one or more volumes of a solution of the metal ion.
17. An anticariogenic composition consisting essentially of phosphopeptides
prepared by the method of claim 1, wherein the composition is substantially free of
phosphopeptides having a molecular weight of less than about 10,000.
The reference relied on by the examiner is:
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