Appeal No. 1997-2517
Application No. 08/155,102
which is an antagonist to the corresponding native gonadotropin
hormone
which expression system comprises an oligonucleotide
sequence encoding a human alpha subunit mutein lacking through
deletion or alteration one or more of amino acids 88-92 of the
native subunit operably linked to control sequences functional in
said host cell.
The examiner relies on the following references:
Reddy et al. (Reddy) 4,840,896 Jun. 20, 1989
Clark et al. (Clark) 4,959,455 Sep. 25, 1990
Morell et al. (Morell), “The Role of Sialic Acid in Determining the Survival of
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pp. 1461-1467 (1971)
Gottlieb et al. (Gottlieb), “Deficient Uridine Diphosphate-N-acetylglucosamine:
Glycoprotein N-Acetylglucosaminyltransferase Activity in a Clone of Chinese
Hamster Ovary Cells with Altered Surface Glycoproteins,” Journal of Biological
Chemistry, Vol. 250, No. 9, pp. 3303-3309 (1975)
Merz et al. (Merz), “Studies of the Specific Role of the Subunits of
Choriogonadotropin for Biological, Immunological and Physical Properties of the
Hormone,” Hoppe-Seyler’s Z. Physiol. Chem., Vol. 360, pp. 1783-1797 (1979)
Fiddes et al. (Fiddes), “The Gene Encoding the Common Alpha Subunit of the
Four Human Glycoprotein Hormones,” J. Mol. Appl. Genet., Vol. 1, pp. 3-18
(1981)
Wallace et al. (Wallace), “Oligonucleotide directed mutagenesis of the human
J-globin gene: a general method for producing specific point mutations in cloned
DNA,” Nucleic Acids Research, Vol. 9, No. 15, pp. 3647-3656 (1981)
Parsons et al. (Parsons), “Purification of an Alternate Form of the I Subunit of
the Glycoprotein Hormones from Bovine Pituitaries and Identification of its
O-Linked Oligosaccharide,” Journal of Biological Chemistry, Vol. 258, No. 1, pp.
240-244 (1983)
Matzuk et al. (Matzuk PNAS), “Effects of preventing O-glycosylation on the
secretion of human chorionic gonadotropin in Chinese hamster ovary cells,”
Proc. Natl. Acad. Sci. USA, Vol. 84, pp. 6354-6358 (1987)
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