Appeal No. 1998-0529
Application No. 07/961,076
(a) using a fucosyltransferase to form an O-glycosidic bond between a
nucleoside 5' -diphospho-fucose and an available hydroxyl group of a carbohydrate
acceptor molecule to yield a fucosylated carbohydrate and a nucleoside 5'-diphosphate,
and;
(b) recycling in situ the nucleoside 5' -diphosphate with fucose to form the
corresponding nucleoside 5' -diphospho-fucose.
The references relied upon by the examiner are:
Wong et al. (Wong) 5,278,299 Jan. 11, 1994
Gokhale et al. (Gokhale) “Chemical Synthesis of GDP-fucose analogs and their
utilization by the Lewis " (1-4) fucosyltransferase,” Cancer Journal Chemical, Vol. 68, pp.
1063-1071 (1989)
Ichikawa et al. (Ichikawa (I)) “Enzyme-Catalyzed Synthesis of Sialyl Oligosaccharide
with in Situ Regeneration of CMP- Sialic Acid,” Journal of the American Chemical Society,
Vol. 113, pp. 4698-4700 (1991)
Ichikawa et al. (Ichikawa (II)) “A Highly Efficient Multienzyme System for the One-
Step Synthesis of a Sialyl Trisaccharide: In Situ Generation of Sialic Acid and N-
Acetyllactosamine Coupled with Regeneration of UDP-Glucose, UDP-Galactose, and
CMP-Sialic Acid,” Journal of the American Chemical Society, Vol. 113, pp. 6300-6302
(1991)
Ground of Rejection2
Claims 1 - 20 stand rejected under 35 U.S.C. § 103. As evidence of
2In setting forth the grounds of rejection at page 4 of the Examiner's Answer, the
examiner has indicated that claims 1-20 stand finally rejected under 35 U.S.C. § 112,
second paragraph. However, at page 3 of the Examiner's Answer, the examiner indicates
that the rejection under 35 U.S.C. § 112, second paragraph has been withdrawn. Since
there is no further reference to this rejection in the Examiner's Answer, we have assumed it
has been withdrawn and have not considered this as an issue in this appeal.
2
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