Ex Parte CRIDDLE et al - Page 3




              Appeal No. 1998-3388                                                                                        
              Application 08/370,551                                                                                      


              the specification as being directed to a method of remediating an environment                               
              contaminated with carbon tetrachloride by introducing Pseudomonas sp. strain KC                             
              (PsKC) into the environment under conditions which permit the microorganism to                              
              convert the carbon tetrachloride, present in the environment being treated, directly into                   
              carbon dioxide and a non-volatile water soluble fraction.                                                   
                                                     Discussion                                                           
                                                  Claim interpretation                                                    
                     Claim 1 is directed to a method of remediating an environment of soil or water,                      
              which has a neutral pH, which contains a diverse microbial population and which is                          
              contaminated with carbon tetrachloride (CT).  The pH of the environment is initially                        
              adjusted to a pH of about 7.8 to 9.2.  A culture of Pseudomonas strain sp. KC (PsKC) is                     
              introduced into the environment to be treated under anaerobic conditions and in a                           
              number and at a temperature sufficient to permit the microorganism to convert the                           
              carbon tetrachloride directly into carbon dioxide and a nonvolatile water soluble fraction.                 
              The claim, further, provides that the PsKC has been grown in a culture medium prior to                      
              introduction into the environment to be treated.                                                            


                                        The rejection under 35 U.S.C. § 102(b)                                            
                     In rejecting claims 1, 3, and 9 - 11, the examiner cites Criddle as teaching                         
              (Answer, pages 3-4):                                                                                        

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