Appeal No. 1999-0111
Application 08/484,593
The references relied upon by the examiner are:
Koumura et al. (Koumura) 4,591,554 May 27, 1986
Carr et al. (Carr) 5,064,756 Nov. 12, 1991
Sussman et al. (Sussman) 5,236,827 Aug. 17, 1993
Claims 1-3, 6, 7, 9-16, 19, 20, 22-28, 31, 32, 34-39, 42, 43 and 45-53 stand
rejected under 35 U.S.C. § 103 as unpatentable over Sussman, Carr and Koumura.
We reverse.
BACKGROUND AND DISCUSSION
The present invention is directed to (i) a method of detecting the presence of
bacteria in a food sample which comprises inoculating a growth medium having a first
substrate which is capable of being acted upon by a phosphatase, a second substrate
which is capable of being acted upon by a glycosidase, and a third substrate which is
capable of being acted upon by a peptidase, with a food product, and (ii) a bacterial
growth medium which comprises a first enzyme substrate for a phosphatase, a second
enzyme substrate for a glycosidase, and a third enzyme substrate for a peptidase, wherein
said enzyme substrates produce a detectable signal when hydrolyzed by their respective
enzyme. In addition, the invention is directed to a method of detecting the presence of
bacteria in a food sample which comprises inoculating a growth medium having a first
substrate which is capable of being acted upon by a glycosidase, and a second substrate
which is capable of being acted upon by a peptidase, with a food product.
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