Appeal No. 1999-0413
Application No. 08/294,819
Claim 1 is illustrative of the subject matter on appeal and is reproduced
below:
1. A rapid analytical method for detection of AJ amyloid formation in a
biological fluid from a human patient suspected of amyloidosis by comparing with
AJ amyloid formation in a biological fluid from a control human subject which
comprises:
(a) preparing a first set of reaction mixtures comprising neat biological
fluid from the control human subject, and serial dilutions of said fluid from the control
subject made in aqueous buffer or physiological solution;
(b) preparing a second set of reaction mixtures comprising the same
type of neat biological fluid from the human patient suspected of amyloidosis and
serial dilutions of said fluid from the patient made in aqueous buffer or physiological
solution;
(c) adding an equal amount of AJ peptide comprising at least amino
acids 6 to 28 of AJ to each serial dilution sample and each neat sample;
(d) contacting each of the first and the second set of reaction mixtures
with an amount greater than 300 nM but less than or equal to 50 µM of an added
heavy metal cation capable of binding to an AJ peptide comprising at least amino
acids 6 to 28 of AJ;
(e) centrifuging each of the first and the second sets of reaction mixtures
to give a first and a second set of pellets, respectively; and
(f) measuring and comparing the amount of amyloid in the first and the
second set of pellets and thereby detecting AJ amyloid formation in the biological
fluid from the human patient suspected of amyloidosis.
The references relied upon by the examiner are:
Maggio et al. (Maggio) 5,434,050 Jul. 18, 1995
Mantyh et al. (Mantyh), “Aluminum, Iron, and Zinc Ions Promote Aggregation of
Physiological Concentrations of ß–Amyloid Peptide,” J. Neurochemistry, Vol. 61,
No. 3, pp. 1171-1174 (1993).
2
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