Appeal No. 1999-1367
Application 08/453,998
variants of said receptors which retain the function of said receptors in yeast,
wherein said receptor comprises a first fragment that recognizes said ligand and a second
fragment that binds to said transcriptional control sequence;
ii) culturing said transformed yeast cell resulting from step (i) in the presence of said
ligand completed with the said expressed receptor whereby said transcription control
sequence is induced and said heterologous protein is thereby produced; and
iii) isolating said heterologous protein.
The references relied upon by the examiner are:
Evans et al. (Evans) 5,071,773 Dec. 10, 1991
Meyhack et al. (Meyhack) 5,175,105 Dec. 29, 1992
Green et al. (Green) “Human Oestrogen Receptor cDNA: Sequence, Expression and
Homology to V-erb-A,” Nature Vol. 320 pp. 134-139 (1986)
Green et al. (Green) “A Versatile in Vitro and in Viro Eukaryotic Expression Vector for
Protein Engineering,” Nucleic Acids Research Vol. 16 No. 1 p.369(1988)
Krust et al. (Krust) “ The Chicken Oestrogen Receptor Sequence: Homology with
V-erbA and the Human Oestrogen and Glucocorticoid Receptors,” The EMBO Journal
Vol.5(5) pp. 891-897 (1986)
West et al. (West) “Saccharomyces Cerevisiae GAL1-GAL10 Divergent Promoter Region:
Location and Function of the Upstream Activating Sequence UAS Molecular and Cellular
G”
Biology Vol. 4(11) pp. 2467-2478 (1984)
Martinez et al. (Martinez) “ The Estrogen-responsive element as an Inducible enhancer:
DNA Sequence requirements and Conversion to a Glucocorticoid-Responsive element,”
The EMBO Journal Vol. 6(12) pp. 3719-3727 (1987)
Cushing et al. (Cushing) “A Study of the Effects of 17-$-Estradiol on The Growth of
Saccharomyces Cerevisiae,” Academy of Science Vol. 79 pp.111-116 (1986)
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