Appeal No. 1999-1730
Application No. 08/478,205
1. An isolated DNA molecule comprising a segment having a
sequence which encodes a synthetic mutant beetle luciferase
comprising an amino acid sequence that difeers from that of the
corresponding wild-type luciferase by at least one amino acid
substitution, the position of the amino acid substitution
corresponding to a position in the amino acid sequence of
LucPplGR of SEQ ID NO: 2 selected from the group consisting of
position 215, 224, 232, 236, 237, 242, 244, 245, 248, 282, 283 and
348, wherein the mutant luciferase produces bioluminescence
having a shift in wavelength of peak intensity of at least 1
nanometer relative to the bioluminescence produced by the wild-
type luciferase.
33. An isolated DNA molecule comprising a segment having a
sequence which encodes a mutant beetle luciferase having an
amino acid sequence that differs from that of the corresponding
wild-type luciferase LucPplGR by at least one amino acid
substitution, wherein the encoded mutant luciferase is selected
from the group consisting of LucPplGR-R223L, -R223Q, -R223M,
-R223H, -L238R, -L238M, L238Q, -L238S, -L238D, -S247H, -S247T, -S257Y,
-S247F and the encoded mutant luciferase produces
bioluminescence having a shift in wavelength of peak intensity of a
least 1 nanometer relative to the bioluminescence produced by the
wild-type luciferase.
The examiner relies on the following references:
Wood (Wood (1990)), “Luc Genes: Introduction of Colour into Bioluminescence
Assays,” Journal of Bioluminescence and Chemiluminescence, Vol. 5, pp. 107-
114 (1990)
Wood (Wood dissertation) “Luciferases of Luminous Beetles: Evolution, Color
Variation, and Applications,” Ph.D. dissertation, University of California at San
Diego, 1989.
Claims 1-17, 20-23, 25-27, 29, and 33 stand rejected under 35 U.S.C.
§ 103 as obvious over the Wood dissertation and Wood (1990).
We reverse.
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