Interference 103,579
GBSS gene expression in potato plants is no less inhibited by
constructs including cDNA segments in the antisense direction
inserted into the potato plant genome than by the corresponding
constructs including genomic DNA segments in the antisense
direction. However, a higher percentage of potato plants with
completely inhibited GBSS gene expression was produced using
antisense cDNA inserts than was produced using corresponding
antisense genomic DNA inserts.
We fail to understand how Hofvander’s homologous sequence
theory of obviousness itself explains why Kuipers’ pKGBA55
construct, including a cDNA fragment in the antisense direction,
a cDNA fragment which corresponds to the SUB25 genomic DNA
fragment in the antisense direction used in Kuipers pKGBA25
construct (VDX 4, p. 749, Fig. 2A-C), completely inhibits potato
GBSS gene expression while Kuipers’ pKGBA25 construct does not
(VDX 4, p. 748, Table 1; VDX 4, p. 749, Fig. 2B.). We also fail
to understand how Hofvander’s homologous sequence theory of
obviousness itself explains why Kuipers’ pKGBA50 constructs
comprising full length cDNA in the antisense direction are 800%
more effective in completely inhibiting PGBSS gene expression
than Kuipers’ pKGBA10 comprising full length genomic DNA in the
antisense direction (VDX 4, p. 749, Fig. 2A).
Of greater significance to the issue before us, however, is
the following discussion of the experimental results for full
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