Interference 103,579
fragments they claim have common sequences which are homologous
to the PGBSS gene to be inhibited in the antisense direction.
Table 1 of Kuipers 1995 publication tabulates (VDX 4,
p. 748; HR 339):
Inhibition of granule-bound starch synthase (GBSS) gene
expression assessed by iodine staining of (micro-)tuber
starch from transgenic 1024-2 clones carrying different
antisense constructs. The antisense constructs pGB50,
pKGBA50 and pKGBA55 are based on the GBSS cDNA. The
other constructs are based on the genomic coding region
of the GBSS gene.
Table 1 is reproduced in its entirety below (VDX 4, p. 748, Table
1; HR 339, Table 1):
Construct Number of Number of Number of
transformants transformants transformants
with inhibition not inhibitiona
CompleteaIncompletea
pGBA10 36 1 (3%) 23 (64%) 12 (33%)
pKGBA10 35 1 (3%) 17 (49%) 17 (49%)
pGBA20 36 0 1 (3%) 35 (97%)
pKGBA20 28 0 2 (7%) 26 (93%)
pKGBA25 49 0 6 (12%) 43 (88%)
pGBA30 87 0 0 87 (100%)
pKGBA30 71 0 1 (1%) 70 (99%)
pKGBA31 41 2 (5%) 21 (51%) 18 (44%)
pKGB50 26 3 (12%) 21 (80%) 2 (8%)
pKGBA50 32 8 (25%) 14 (44%) 10 (31%)
pKGBA55 48 2 (4%) 2 (4%) 44 (92%)
a Complete inhibition: starch granules showing red staining
starch with a small blue staining core after iodine staining.
Incomplete inhibition: starch granules showing a medium sized
or large blue staining core and a red staining outer part of
the granule after iodine staining. No inhibition: starch
granules showing blue staining starch after iodine staining.
Figure 1B defines the constructs indicated in Table 1 as follows
(promoter (35S or GB) and either full length genomic DNA coding
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