Interference 103,579
achieved using any particular fragment thereof in the antisense
direction. Compare the successful results using Kuipers’ pKGBA10
constructs comprising SUB10 in the antisense direction to the
unsuccessful results using Kuipers’ pKGBA20 and pKGBA25
constructs respectively comprising SUB20 and SUB25 genomic DNA
fragments of SUB10 full length genomic DNA coding for PGBSS in
the antisense direction.
Long after the effective filing dates of Hofvander’s and
Visser’s involved applications, Kuipers’ 1995 publication
reported that, while the knowledge of persons skilled in the art
of antisense technology had greatly increased since 1988, still
(VDX 4, pp. 752-754 [sic 753], bridging para.):
. . . variation[s] in the inhibitory effects of the
partial genomic antisense constructs [can no more than]
point . . . towards a function for certain regions of
the gene in antisense inhibition.
According to Kuipers’ 1995 publication, most recently reported
studies in the art “might indicate that certain sequence
characteristics are involved in the process of antisense
inhibition” (VDX 4, p. 752, col. 2, final incomplete para.;
emphasis added). On the other hand, they might not (VDX 4,
pp. 752-754 [sic 753], bridging para.). Alas, Kuipers’ 1995
publication points to evidence that “might indicate, that in
contrast to what has often been hypothesized” (VDX 4, p. 754
[sic 753], col. 1, last sentence of the first incomplete para.),
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