Interference No. 104,021 Paper No. 112
Griffin v. Bertina Page 6
F12. Jose Fernandez, another colleague of the Griffin inventors, performed a
coagulation assay of the S family blood and determined that a sample from S family member
"LS" exhibited APC resistance, while a sample from S family member "AS" did not (G035-36;
Exhs. 1030 & 1031).
F13. On 27 August 1993, Xu amplified DNA samples from S family members,
including AS and LS, using the eight primers (FV1-FV8A)4 as well as additional primers (FV9-
FV12)5 (G032; Exh. 1021).
F14. On 18 October 1993, Xu prepared sequencing gels (Exh. 1023)6 for LS and for AS
(G033; Exh. 1022) using the FV7 primer, among others. The record copy of the 18 October 1993
gel contains no indication that the nt 1691 point mutation was recognized. Exhibit 1023
indicates that AS only has a band in the G column for nucleotide 1691.
F15. On 25 October 1993, Xu again prepared sequencing gels (Exh. 1024)7 for LS and
for AS (Exh. 1022 at 3) using the FV7 primer, among others. The record copy of the 25 October
1993 gel contains no indication that the nt 1691 point mutation was recognized. Exhibit 1024
indicates that AS only has a band in the G column for nucleotide 1691.
F16. The gels lanes are in sets of four for A, C, G, and T, respectively, running left to
right. The sequence should be read up from the bottom.
4 Griffin's record is inconsistent in its labeling of primers. Some primers, such as FV8 variously include either
an "A" or "a" designation. We assume that, for the purposes of our decision FV8, FV8A, and FV8a are the same. The
critical sequencing gels in the record were made using only the FV7 primer in any case.
5 The primers sometimes have an "A" or "a" added to their names. If this addition indicates a different primer,
then even more primers may have been tested.
6 Exhibit 1025 is an enlarged and labeled portion of Exhibit 1023.
7 Exhibit 1026 is an enlarged and labeled portion of Exhibit 1024.
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