Interference No. 104,021 Paper No. 112
Griffin v. Bertina Page 8
F21. While the testimony indicates that the Griffin inventors had discovered a
noteworthy mutation, none of this testimony states that the inventor's recognized that they had
discovered the mutation that caused the condition they were studying.
F22. A page of notes (Exh. 1032) for a lab meeting on 2 December 1993 attributed to
Greengard and Xu discuss the use of the FV1-FV12 primers on eight APC functional sites in the
S family. They report that "[n]ot all of these have been explored in the [S] fa[m]ily as yet." The
notes show that primers FV7/8a correspond to an N-terminal APC cleavage site (Exh. 1032).
F23. The notes report (Exh. 1032) the discovery of five10 nucleotide mutations as
follows:
Nucleotide Effect on protein
2298 Silent
2325 Silent
5380 Silent
5248 (A T) Serine Cysteine
5380 (G A) Valine Methionine
F24. Both of the protein changes caused by mutations are in the phospholipid binding
site, which is probed using primers FV3/4 (Exh. 1032).
10 The notes indic ate two mutations at nu cleotide 5380, one that does not change the encoded a mino acid
(silent) and one that does.
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